The study was designed to investigate the availability and use of reference information sources by undergraduate students. Also, attempt was made to ascertain the extent of use and benefit of reference information source to the students. Data was collected through the use of questionnaire administered on 102 samples randomly drawn from the Ambrose Alli University and University of Benin. The data were analysed using simple percentage. Findings revealed that prominent reference information sources like encyclopaedia, abstracts, dictionaries, bibliographies, index and almanac are more in these institutions’ libraries. Result showed that a relationship exist between the users of reference information and reference librarians in the library. It was found that the extent of use of reference materials is still relatively low especially among student users. Materials such as government documents, year book, abstract, atlas, maps, and almanac are rarely utilised. Meanwhile, the reference information sources available in the institutions served useful purpose. The respondents highly acknowledge their benefits especially reference materials like bibliographies, index, encyclopaedia, dictionaries, yearbooks and handbooks. Lastly, result showed that there are many problems associated with the use of reference information sources by undergraduate students amongst which are: ignorance or lack of knowledge about library reference service, ill attitude of reference librarians, lack of indigenous materials, lack of funds, and poor power supply. In the light of the above, conclusion was drawn and it was recommended that efforts should be made to acquire specific information sources that the users need but not available in the library.
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